We narrowed to 14,156 results for: ckb

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pAW8ENdeI-cyEGFP-4XDSR

Plasmid

#49864

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-8XDSR

Plasmid

#49871

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-4XDSR

Plasmid

#49869

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-A

Plasmid

#41476

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-G

Plasmid

#41478

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTAL13

Plasmid

#37186

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Tags

FokI

Expression

Mammalian

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEMY07AB-ORF

Plasmid

#127711

Purpose

Level 0 destination vector for a coding sequence with B - C scars. Clone in with BpiI

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pEMY07CD-TER

Plasmid

#127712

Purpose

Level 0 destination vector for a terminator with C - D scars. Clone in with BpiI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-5a

Plasmid

#127716

Purpose

Level 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-ab

Plasmid

#127717

Purpose

Level 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-bc

Plasmid

#127718

Purpose

Level 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-cd

Plasmid

#127719

Purpose

Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-de

Plasmid

#127720

Purpose

Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-ef

Plasmid

#127721

Purpose

Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pDSM-f3

Plasmid

#127722

Purpose

Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pAW

Plasmid

#133893

Purpose

Expression of Gal4AD-Prey hybrid protein

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pBW

Plasmid

#133897

Purpose

Expression of Gal4BD-Bait hybrid protein.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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