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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  2. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid
    #223110
    Purpose
    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpG-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223110-plasmid-map-sequence-id-447633

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  13. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

    Plasmid
    #193037
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, L355A, Residues 316-366, Non-native C-term…
    Promoter
    T7
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193037-plasmid-map-sequence-id-379006

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Showing: 9881 - 9900 of 19078 results