We narrowed to 20,324 results for: Sele
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Plasmid#149270PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRC/CMV-JAK1K908E-VSV
Plasmid#139357PurposeExpression of kinase-dead JAK1-K908E proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
TagsVSVExpressionMammalianMutationK908EPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-Y766F
Plasmid#59777PurposeExpresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1DepositorAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/TO-myc-BirA*-NES
Plasmid#112714PurposeFor tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIV Rev nuclear export signal (NES)
ExpressionMammalianAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
TagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev(T/A)
Plasmid#118479PurposeNegative-control mutant for RAB-AKARev biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB-AKARev(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-BAD
Plasmid#113459PurposeProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBCL2 associated agonist of cell death (BAD Human)
TagsProteinA-mCitrineExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-FHA1
Plasmid#87711PurposeEncodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDronpa-FHA1
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKAsub-TagRFPT-CAAX
Plasmid#87712PurposeEncodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKAsub-TagRFPT-CAAX
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-DEVD-TagRFPT
Plasmid#87704PurposeFluorescent biosensor for super-resolution imaging of caspase activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDronpa-DEVD-TagRFPT
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK TT 3xFLAG Luciferase neo
Plasmid#98392PurposeLentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tagDepositorInsertLuciferase
UseLentiviralTags3xFLAGExpressionMammalianAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP3-44
Plasmid#91780PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP3-44
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE4566
Plasmid#88904PurposeExpresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nucleaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsMb crRNA
hMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSc1
Plasmid#80436PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertssp Cas9 gRNA
eGFP
UseCRISPRExpressionMammalianPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cry2 mCherry PGK-1 in pcDNA3.1
Plasmid#64214PurposemCherry fused between Cry2 and PGK-1 to study cell migrationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmcherryExpressionMammalianPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 299/399 dual snp flag pDEST40
Plasmid#42648Purposedestination vector for expression of human TLR4 299/399 SNP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTLR4 299/399 SNP (TLR4 Human)
TagsFlagExpressionMammalianMutationD299G and T399IPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (del.Cyto)
Plasmid#49209Purposeexpresses Flag tagged mouse Tim3 fragment (no cytosolic domain)DepositorInsertTim3 (del.Cyto) (Havcr2 Mouse)
TagsCD8 signal sequence and FLAGExpressionMammalianMutationcytosolic domain deleted, no Tim3 signal sequence…PromoterEf1aAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-(925-1709)
Plasmid#42004DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationcontain aa925-1709PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only