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Showing: 161 - 200 of 11183 results

  2. (+)-Limonene Synthase

    Plasmid
    #112230
    Purpose
    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
    Depositor
    Daniel Oprian
    Article
    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17
    Insert
    (+)-Limonene Synthase
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-52
    Promoter
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112230-plasmid-map-sequence-id-214310

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  6. pGL3_zfh1_CP-candidate_luc+

    Plasmid
    #86391
    Purpose
    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Promoter
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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  9. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

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  10. pSTAP-seq_fly-tj

    Plasmid
    #86386
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster tj enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86386-plasmid-map-sequence-id-173933

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  11. pSTAP-seq_fly-ssp3

    Plasmid
    #86382
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ssp3 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86382-plasmid-map-sequence-id-173943

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  12. pSTAP-seq_fly-ham

    Plasmid
    #86383
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ham enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86383-plasmid-map-sequence-id-173939

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  13. pSTAP-seq_fly-sgl

    Plasmid
    #86384
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster sgl enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86384-plasmid-map-sequence-id-173940

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  14. pSTAP-seq_fly-ncm

    Plasmid
    #86385
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ncm enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86385-plasmid-map-sequence-id-173945

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  20. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107454-plasmid-map-sequence-id-207518

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  21. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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  22. pLX304_miRFP670nano-P2A-ERM-gCam6f

    Plasmid
    #214927
    Purpose
    Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)
    Depositor
    Xinnan Wang
    Article
    Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi
    Insert
    miRFP670nano-P2A-ERM-gCam6f
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMVp
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    214927-plasmid-map-sequence-id-429502

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  27. pBAD-pHScarlet

    Plasmid
    #191258
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet
    Depositor
    Robert Campbell
    Insert
    pHScarlet
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191258-plasmid-map-sequence-id-374867

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  28. pBAD-pHmGL

    Plasmid
    #191257
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHmGL
    Depositor
    Robert Campbell
    Insert
    pHmGL
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    191257-plasmid-map-sequence-id-378487

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  29. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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  30. Lmir_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228385
    Purpose
    Expresses N-term transit peptide from Lophophytum mirabile cytoplasmic Glutamate aminoacyl-tRNA synthetase (TRINITY_DN2194_c0_g1_i3); determination of L. mirabile cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. mirabile cytoplasmic GluRS (TRINITY_DN2194_c0_g1_i3)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228385-plasmid-map-sequence-id-455876

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  31. Lmir_orgGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228386
    Purpose
    Expresses N-term transit peptide from Lophophytum mirabile organellar Glutamate aminoacyl-tRNA synthetase (TRINITY_DN6_c0_g3_i1); determination of L. mirabile organellar GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. mirabile organellar GluRS (TRINITY_DN6_c0_g3_i1)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228386-plasmid-map-sequence-id-455877

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  32. Lmir_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228387
    Purpose
    Expresses N-term transit peptide from Lophophytum mirabile glutamyl-tRNA reductase (TRINITY_DN3595_c0_g2_i6); determination of L. mirabile glutamyl-tRNA Reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. mirabile glutamyl-tRNA reductase (TRINITY_DN3595_c0_g2_i6)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228387-plasmid-map-sequence-id-461175

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  33. Lpyr_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228388
    Purpose
    Expresses N-term transit peptide from Lophophytum pyramidale cytoplasmic Glutamate aminoacyl-tRNA synthetase (TRINITY_DN180208_c0_g1_i5); to determine L. pyramidale cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. pyramidale cytoplasmic GluRS (TRINITY_DN180208_c0_g1_i5)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228388-plasmid-map-sequence-id-455878

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  34. Lpyr_orgGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228389
    Purpose
    Expresses N-term transit peptide from Lophophytum pyramidale organellar Glutamate aminoacyl-tRNA synthetase (TRINITY_DN1690_c0_g1_i10); to determine L. pyramidale organellar GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. pyramidale organellar GluRS (TRINITY_DN1690_c0_g1_i10)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228389-plasmid-map-sequence-id-455879

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  35. Lpyr_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228390
    Purpose
    Expresses N-term transit peptide from Lophophytum pyramidale glutamyl-tRNA reductase (TRINITY_DN24865_c0_g2_i5); determination of L. pyramidale glutamyl-tRNA reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. pyramidale glutamyl-tRNA reductase (TRINITY_DN24865_c0_g2_i5)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228390-plasmid-map-sequence-id-461829

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  36. Balfun_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228382
    Purpose
    Expresses N-term transit peptide from Balanophora fungosa cytoplasmic Glutamate aminoacyl-tRNA synthetase (GWHDONK00049274); determination of B. fungosa cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from B. fungosa cytoplasmic GluRS (GWHDONK00049274)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228382-plasmid-map-sequence-id-455874

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  37. Balfun_orgGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228383
    Purpose
    Expresses N-term transit peptide from Balanophora fungosa organellar Glutamate aminoacyl-tRNA synthetase (GWHTDONK003589); determination of B. fungosa organellar GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from B. fungosa organellar GluRS (GWHTDONK003589)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228383-plasmid-map-sequence-id-455875

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  38. Balfun_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228384
    Purpose
    Expresses N-term transit peptide from Balanophora fungosa glutamyl-tRNA reductase (GWHDONK00038241); determination of B. fungosa glutamyl-tRNA Reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from B. fungosa glutamyl-tRNA reductase (GWHDONK00038241)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228384-plasmid-map-sequence-id-461174

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  39. pSBBi-RP TCS1

    Plasmid
    #139161
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase 1 [Camellia sinensis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139161-plasmid-map-sequence-id-309127

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  40. pSBBi-RP CsAncCS

    Plasmid
    #139167
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Camellia sinensis Ancestral Caffeine Synthase
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139167-plasmid-map-sequence-id-309357

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Showing: 161 - 200 of 11183 results