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Showing: 201 - 250 of 475 results

  12. pKD239-1g-2

    Plasmid
    #90959
    Purpose
    Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bba_J23100, Bba_J23109, and PttrB185-269
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90959-plasmid-map-sequence-id-178329

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  13. pKD233.7-3

    Plasmid
    #90955
    Purpose
    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bba_J23114, PltetO-1, and PttrB185-269
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90955-plasmid-map-sequence-id-178326

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  19. TFORF1317

    Plasmid
    #143788
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RB1CC1 (RB1CC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143788-plasmid-map-sequence-id-396238

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  20. TFORF2651

    Plasmid
    #144058
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TERB1 (TERB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144058-plasmid-map-sequence-id-400556

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  27. TFORF1316

    Plasmid
    #143787
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RB1CC1 (RB1CC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143787-plasmid-map-sequence-id-396239

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  28. pEUI(+)

    Plasmid
    #111834
    Purpose
    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Article
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SV40, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111834-plasmid-map-sequence-id-218213

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  31. pENTR-EUI

    Plasmid
    #111835
    Purpose
    Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Article
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Entry vector for expression
    Tags
    Expression
    Mutation
    Promoter
    CMV, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111835-plasmid-map-sequence-id-218214

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  32. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  33. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  34. pRS415-KEX2

    Plasmid
    #10996
    Depositor
    Francisco Malagon
    Insert
    KEX2 (KEX2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10996-plasmid-map-sequence-id-6831

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  35. R777-E305 Hs.SCRIB

    Plasmid
    #70589
    Purpose
    Gateway ORF clone of human SCRIB [NM_182706.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SCRIB (SCRIB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70589-plasmid-map-sequence-id-133940

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  36. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  37. R777-E171 Hs.PIK3R1

    Plasmid
    #70455
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70455-plasmid-map-sequence-id-125904

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  38. R777-E306 Hs.SCRIB-nostop

    Plasmid
    #70590
    Purpose
    Gateway ORF clone of human SCRIB [NM_182706.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SCRIB (SCRIB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70590-plasmid-map-sequence-id-133941

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  39. R777-E172 Hs.PIK3R1-nostop

    Plasmid
    #70456
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70456-plasmid-map-sequence-id-125905

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  40. pLenti4/V5-DEST-P85(G376R)

    Plasmid
    #40220
    Depositor
    Lynda Chin
    Insert
    P85(G376R) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Glycine 376 to Arginine
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only

  41. pLenti4/V5-DEST-P85(W583del)

    Plasmid
    #40229
    Depositor
    Lynda Chin
    Insert
    P85(W583del) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deleted amino acid 583
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  42. pLenti4/V5-DEST-P85(N564K)

    Plasmid
    #40226
    Depositor
    Lynda Chin
    Insert
    P85(N564K) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Asparagine 564 to Lysine
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  43. pLenti4/V5-DEST-P85(T576del)

    Plasmid
    #40228
    Depositor
    Lynda Chin
    Insert
    P85(T576del) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deleted amino acid 576
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  45. pLenti4/V5-DEST-P85(E439del)

    Plasmid
    #40221
    Depositor
    Lynda Chin
    Insert
    P85(E439del) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deleted amino acid 439
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only

  46. SEPX1

    Plasmid
    #39048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEPX1 (MSRB1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys95-S
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. BIRC1A

    Plasmid
    #42400
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BIRC1A (NAIP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pLenti4/V5-DEST-P85(KS459del)

    Plasmid
    #40222
    Depositor
    Lynda Chin
    Insert
    P85(KS459delN) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    amino acids 459-460 replaced with asparagine
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only

  49. pLenti4/V5-DEST-P85(DKRMNS560del)

    Plasmid
    #40225
    Depositor
    Lynda Chin
    Insert
    P85(DKRMNS560del) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deleted amino acids 560-565
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  50. pLenti4/V5-DEST-P85(D560Y)

    Plasmid
    #40223
    Depositor
    Lynda Chin
    Insert
    P85(D560Y) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 560 to Tyrosine
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 250 of 475 results