We narrowed to 7,543 results for: PAC

1. pL-CRISPR.EFS.PAC

   Plasmid

   #57828

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAC1749

   Plasmid

   #127983

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACDura3

   Plasmid

   #138711

   Purpose

   Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   ura3 gene (URA3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   optimized ura3 gene for expression in Escherichia…

   Promoter

   pEM7 promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pACYCDuet_WTDHFR_WTTS

   Plasmid

   #91226

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRPaCas9

   Plasmid

   #111819

   Purpose

   Expresses Cas9 in T. brucei (2T1) cells

   Depositor

   David Horn

   Article

   Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC14

   Plasmid

   #197408

   Purpose

   Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

   Depositor

   Yolanda Schaerli

   Article

   Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

   Insert

   pTF-FC2 replicon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS_2paCRY2

   Plasmid

   #129651

   Purpose

   2P-activatable CRY2

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pACT2.2

    Plasmid

    #11343

    Depositor

    Guy Caldwell

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC37

    Plasmid

    #111618

    Purpose

    Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

    Depositor

    Tim Tolker-Nielsen

    Article

    Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

    Insert

    PpqsA-gfp(ASV)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACT2.2gtwy

    Plasmid

    #11346

    Depositor

    Guy Caldwell

    Insert

    gateway cassette
    

    Use

    Tags

    Gal4 Activation Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACYC.GFP1Amb

    Plasmid

    #102792

    Purpose

    Expresses GFP with a premature stop codon

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y39Stop

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACU

    Plasmid

    #58373

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Yuh-Nung Jan, Chun Han

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACS130

    Plasmid

    #204595

    Purpose

    Histone H3.1K9M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1K9M-V5

    Expression

    Mammalian

    Mutation

    H3.1K9M

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_PACSIN2-1/1

    Plasmid

    #91330

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN2-1/1 (PACSIN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge