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Plasmid#232744PurposeExpresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.DepositorInsertNAPstar7
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPoptim
Plasmid#221088PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPoptim
Plasmid#221089PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPorig
Plasmid#221091PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPoptim
Plasmid#221093PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPoptim
Plasmid#221094PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPorig
Plasmid#221086PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPorig
Plasmid#221087PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYP-ptef1-GFP
Plasmid#199990PurposeYeast/E coli shuttle vector expressing GFP from tef1 promoterDepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-StefinA
Plasmid#182418PurposeBacterial expression of mNeongreen-stefinA fusionDepositorInsertmNeongreen-stefin (CSTA Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
UseTagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS405-LEU2-TEF-KpolDcr1v2
Plasmid#85193PurposeIntegrating plasmid, K. polysporus Dcr1 under TEF promoterDepositorInsertDcr1
UseIntegrating, shuttle vectorTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(272)
Plasmid#44375DepositorInsertUAS1B16-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(272)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(272)
Plasmid#44376DepositorInsertUAS1B8-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(272)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits