We narrowed to 2,195 results for: Xpa

1. pCS2-EcYRS-EGFP*

   Plasmid

   #191118

   Purpose

   A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
   

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvol-MmKlacRS1

   Plasmid

   #232157

   Purpose

   Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   KlacRS1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. gCOTS-pyl

   Plasmid

   #92048

   Purpose

   This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Cyanobacteria s. elongatus genome recombination i…

   Promoter

   LeuP (native S. elongatus promoter) and PrcbL

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEvol-chKlacRS-WT

   Plasmid

   #232159

   Purpose

   A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   chKlacRS-WT
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAL4-DBD-RUNX2+10A-IDR

   Plasmid

   #145251

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2A+10A-IDR
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvol-chKlacRS-IPYE

   Plasmid

   #232160

   Purpose

   A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   chKlacRS-IPYE
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-MfnG-EcYRS-EGFP*

   Plasmid

   #191119

   Purpose

   A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
   

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

   Plasmid

   #174896

   Purpose

   Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

    Plasmid

    #174899

    Purpose

    Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid

    #140022

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    PcKRS (pylS Methanosarcina bakeri)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M241F, A267S, Y271C, L274M

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid

    #140019

    Purpose

    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoLeuRS (AnapRS)
    

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid

    #140021

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145268

    Purpose

    Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-HOXD13(+7A)-IDR-mCherry

    Plasmid

    #145281

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

19. RCASBP(A)-3xFlag-mHoxd13+7Ala

    Plasmid

    #145289

    Purpose

    Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    Hoxd13(+7Ala) (Hoxd13 Mouse)
    

    Use

    Viral avian expression

    Tags

    3xFlag

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-45b-RUNX2(+10A)-IDR-mCherry

    Plasmid

    #145287

    Purpose

    Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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