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We narrowed to 6,562 results for: nls

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Showing: 81 - 100 of 6562 results

  2. pAAV-CAG-NLS-GFP (AAV PHP.eB)

    Viral prep
    #104061-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    CAG
    Tags
    GFP
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only

  4. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)

    Viral prep
    #51085-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

    Viral prep
    #51085-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)

    Viral prep
    #51083-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (#51083). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato plasmid DNA. Cre-dependent GCaMP6f calcium sensor and physically separate nuclear tdTomato, under control of the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    EF1a
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    June 7, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. xCas9(3.7)-BE3

    Plasmid
    #108380
    Purpose
    Mammalian expression vector for xCas9(3.7)-BE3
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.7)-BE3
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108380-plasmid-map-sequence-id-217617

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  9. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)

    Viral prep
    #51084-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)

    Viral prep
    #51082-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    EF1a
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  11. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42333-plasmid-map-sequence-id-59273

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  14. dxCas(3.7)-VPR

    Plasmid
    #108383
    Purpose
    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dxCas(3.7)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    108383-plasmid-map-sequence-id-207172

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  17. pKA-207I10

    Plasmid
    #79845
    Purpose
    Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
    Expression
    Mammalian
    Mutation
    RpPpsR2 cysteine 439 changed to serine
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    79845-plasmid-map-sequence-id-149588

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  18. pmKalama1-Nuc

    Plasmid
    #14894
    Purpose
    Mammalian expression of mKalama1 blue fluorescent protein with NLS
    Depositor
    Robert Campbell
    Publication
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmKalama1-Nuc
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    I178T
    Promoter
    CMV
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  19. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid
    #61593
    Purpose
    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
    Depositor
    Feng Zhang
    Publication
    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    HA, NLS, and OLLAS tag
    Expression
    Mammalian
    Mutation
    K175R and K736R (deUb mutations)
    Available since
    Feb. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    61593-plasmid-map-sequence-id-470051

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Showing: 81 - 100 of 6562 results