We narrowed to 7,711 results for: nls

1. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107307

   Purpose

   Encoding an SPH activation system

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

   Plasmid

   #69232

   Purpose

   Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

   Plasmid

   #132404

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS

   Plasmid

   #191367

   Purpose

   Overexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dMisCas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

   Plasmid

   #191369

   Purpose

   Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dPba3Cas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

   Plasmid

   #191368

   Purpose

   Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dHgm4Cas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

   Plasmid

   #69233

   Purpose

   R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

   Plasmid

   #69231

   Purpose

   Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 TALE
   

   Use

   CRISPR

   Tags

   2X NLS and NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBPnlsLexA::GADflUw

   Plasmid

   #26232

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::GADfl
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

    Plasmid

    #81251

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR'CMVGFP_NLS_hRIF1(1-1308)

    Plasmid

    #23134

    Depositor

    Lifeng Xu

    Article

    Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

    Insert

    RIF1 deletion construct (RIF1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNLS-iRFP713

    Plasmid

    #45468

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPT-nlsLacZ

    Plasmid

    #12261

    Depositor

    Didier Trono

    Insert

    LacZ
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNLS-iRFP670

    Plasmid

    #45466

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Use

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBPnlsLexA::p65Uw

    Plasmid

    #26230

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::p65 (RELA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-NLS-HA-B3

    Plasmid

    #51270

    Purpose

    Expression of the site-specific recombinase B3 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-B3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJIT163-2NLSCas9

    Plasmid

    #53064

    Purpose

    Expression of rice codon-optimized Cas9 in plant cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    Cas9
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Rice codon optimized

    Promoter

    2x35S

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge