Purpose
Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
Inserts
Cas9
Puromycin resistance
EGFP sgRNA 4
Use
CRISPR and Lentiviral
Available Since
March 11, 2014
Availability
Academic Institutions and Nonprofits only