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Showing: 81 - 100 of 1185 results

  4. pENTR4 CDS A. thaliana PARP1

    Plasmid
    #175818
    Purpose
    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Insert
    PARP1 (PARP1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    one silent mutation, annotated in GenBank file
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175818-plasmid-map-sequence-id-346172

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  7. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  8. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  9. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  10. CRY2olig-PR

    Plasmid
    #218917
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence
    Depositor
    Tatyana Shelkovnikova
    Insert
    CRY2olig-mCherry tagged poly-PR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218917-plasmid-map-sequence-id-442219

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  11. CRY2olig-GP

    Plasmid
    #218918
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence
    Depositor
    Tatyana Shelkovnikova
    Insert
    CRY2olig-mCherry tagged poly-GP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218918-plasmid-map-sequence-id-442210

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  12. CRY2olig-GR

    Plasmid
    #218919
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence
    Depositor
    Tatyana Shelkovnikova
    Insert
    CRY2olig-mCherry tagged poly-GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218919-plasmid-map-sequence-id-442209

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  15. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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  17. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid
    #203510
    Purpose
    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    poly SLEV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203510-plasmid-map-sequence-id-402633

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Showing: 81 - 100 of 1185 results