We narrowed to 6,809 results for: poly

1. FRB-polySH33-EGFP

   Plasmid

   #175792

   Purpose

   Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSc polyA + Shal1

   Plasmid

   #16196

   Depositor

   Larry Salkoff

   Article

   Wei et al Science. 1990 May 4. 248(4955):599-603.

   Insert

   Drosophila melanogaster Shaker cognate l (Shal Fly)
   

   Use

   Xenopus oocyte expression

   Mutation

   N/A

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

4. HB901: pMVP (L3-L2) polyA

   Plasmid

   #121746

   Purpose

   pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Polyadenylation signal (polyA)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSc polyA + Shal2

   Plasmid

   #16144

   Depositor

   Larry Salkoff

   Article

   Butler et al Science. 1989 Feb 17. 243(4893):943-7.

   Insert

   Shal (Shal Fly)
   

   Use

   Xenopus oocyte expression

   Mutation

   N/A

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

6. GLN, Linear-3, PolyU(1)

   Plasmid

   #166984

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1)
   

   Expression

   Mammalian

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97310

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-HIRAmut-polyA

   Plasmid

   #59782

   Purpose

   This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Expression

   Mammalian

   Mutation

   Change from 'GATCGAAGTTTG' to 'GAc…

   Promoter

   CMV and T7

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. P63-sp64 Poly A

    Plasmid

    #59435

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 63
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. P21-sp64 Poly A

    Plasmid

    #59433

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 21
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

    Plasmid

    #226767

    Purpose

    vector with NGFR and GFP

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. poly H35S SUMO1

    Plasmid

    #221071

    Purpose

    Expression of poly H35S SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35S

    Promoter

    tac

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. poly E67R SUMO1

    Plasmid

    #221072

    Purpose

    Expression of poly E67R SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    E67R

    Promoter

    tac

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. poly H35K SUMO1

    Plasmid

    #221070

    Purpose

    Expression of poly H35K SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35K

    Promoter

    tac

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. poly H35R SUMO1

    Plasmid

    #221069

    Purpose

    Expression of poly H35R SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35R

    Promoter

    tac

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGF-bGH polyA-G

    Plasmid

    #218902

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    bGH polyA
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcBAC3-POLY-pnRFP

    Plasmid

    #201680

    Purpose

    Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

    Depositor

    Huiwang Ai

    Article

    Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

    Insert

    813
    

    Expression

    Mammalian

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK005 ubi:mCerulean_ubb-polyA

    Plasmid

    #195979

    Purpose

    Expression construct

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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