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We narrowed to 49,426 results for: prot

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Showing: 81 - 100 of 49426 results

  1. HaloTag-G protein gamma-1

    Plasmid
    #67038
    Purpose
    G protein gamma-1 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-1 (GNGT1 Bovine)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67038-plasmid-map-sequence-id-115574

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  2. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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  3. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    TEV protease
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

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  4. G protein alpha-s-HaloTag

    Plasmid
    #66993
    Purpose
    G protein alpha-s internally tagged with HaloTag and EE epitope
    Depositor
    Catherine Berlot
    Insert
    G-alpha-s-EE-HaloTag (Gnas Rat)
    Tags
    HaloTag flanked by SGGGGSGGGGS on each side was i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66993-plasmid-map-sequence-id-122192

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  5. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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  6. G protein alpha-q-YFP

    Plasmid
    #55782
    Purpose
    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-q EE YFP (Gnaq Mouse)
    Tags
    The EE epitope was introduced into the alpha-q se…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    55782-plasmid-map-sequence-id-119295

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  7. G protein alpha-s-YFP

    Plasmid
    #55781
    Purpose
    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-s-EE YFP (Gnas Rat)
    Tags
    EYFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    Met was substituted for Gln69 in EYFP (Clontech).…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55781-plasmid-map-sequence-id-119279

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  9. HaloTag-G protein beta-1

    Plasmid
    #67016
    Purpose
    G protein beta-1 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein beta-1 (GNB1 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67016-plasmid-map-sequence-id-115474

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  10. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105788-plasmid-map-sequence-id-203379

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  11. LHP1481 - TOPO-ProteinA

    Plasmid
    #32225
    Depositor
    Linda Hicke
    Insert
    Protein A
    Use
    Cloning
    Promoter
    P-lac
    Available Since
    Oct. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55776-plasmid-map-sequence-id-112541

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  13. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  14. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  17. HaloTag-G protein beta-2

    Plasmid
    #67017
    Purpose
    G protein beta-2 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    NM_005273.3 (GNB2 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67017-plasmid-map-sequence-id-115479

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  18. HaloTag-G protein gamma-12

    Plasmid
    #67043
    Purpose
    G protein gamma-12 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-12 (GNG12 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    67043-plasmid-map-sequence-id-115589

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  19. HaloTag-G protein gamma-5

    Plasmid
    #67039
    Purpose
    G protein gamma-5 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-5 (GNG5 Bovine)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67039-plasmid-map-sequence-id-115581

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  20. HaloTag-G protein gamma-7

    Plasmid
    #67040
    Purpose
    G protein gamma-7 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-7 (GNG7 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67040-plasmid-map-sequence-id-115582

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Showing: 81 - 100 of 49426 results