We narrowed to 1,873 results for: sgrna vector

1. pSCAR_sgRNA_hygro-tagBFP-lox2272

   Plasmid

   #162078

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCAR_sgRNA_blast-tagBFP-lox2272

   Plasmid

   #162071

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCAR_sgRNA_puro-mKate-lox5171

   Plasmid

   #162077

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L1p6_barley_sgRNA_scaffold

   Plasmid

   #231033

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1p5_barley_sgRNA_scaffold

   Plasmid

   #231032

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L1p4_barley_sgRNA_scaffold

   Plasmid

   #231031

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L1p3_barley_sgRNA_scaffold

   Plasmid

   #231030

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EC0_6_sgRNA

   Plasmid

   #163717

   Purpose

   Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   sgRNA expression cassette
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 234)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF005-sgRNA-shuffle-in

   Plasmid

   #104441

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-U6-sgRNA-BFP-Puro

    Plasmid

    #229013

    Purpose

    Perturb Seq sgRNA vector with PiggyBac backbone

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLR5-U6-sgRNA-EF

    Plasmid

    #82635

    Purpose

    piggyBac vector for expressing modified sgRNA in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Type

    Empty backbone

    Use

    CRISPR; Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p103DestTol2pA2-4xU6sgRNA

    Plasmid

    #227778

    Purpose

    Destination vector with U6 driven 4 sgRNAs

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid

    #62131

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

    Plasmid

    #62128

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid

    #62127

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge