We narrowed to 1,124 results for: Amot;

1. pCAGmCherry-gRNA

   Plasmid

   #87110

   Purpose

   gRNA cloning vector with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

   Plasmid

   #190236

   Purpose

   AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CTCF-mAC donor (Neo)

   Plasmid

   #140645

   Purpose

   CTCF tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIP-GFP-CERT(PHD)(W33A)

   Plasmid

   #221761

   Purpose

   Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–116, mutated 33W to 33A

   Promoter

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-HA-mBec1 F121A

   Plasmid

   #99506

   Purpose

   Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

   Depositor

   Congcong He

   Article

   Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

   Insert

   Beclin 1 (Becn1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   F121A

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK-VIM270-gSM

   Plasmid

   #215870

   Purpose

   This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

   Depositor

   Kumiko Ui-Tei

   Article

   Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

   Insert

   3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK-VIM270-gCM

   Plasmid

   #215868

   Purpose

   This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

   Depositor

   Kumiko Ui-Tei

   Article

   Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

   Insert

   target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid

    #181997

    Purpose

    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115(S240A) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 240 changed to alanine in p115.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-hWIPI4-3×FLAG

    Plasmid

    #215512

    Purpose

    Expresses FLAG tagged human WIPI4.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECE-M2-AMPKalpha2 M93G

    Plasmid

    #31653

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pECE-HA-AMPKalpha2 M93G

    Plasmid

    #31655

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. EGFP-plk1-AKAP

    Plasmid

    #132967

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C terminus of full-length Plk1 fused to the peric…

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. DHC1 CRISPR

    Plasmid

    #140545

    Purpose

    DHC1 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECE-M2-CDC27 S379A

    Plasmid

    #31662

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog S379A (CDC27 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 379 to Alanine

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-CDC27 S379A

    Plasmid

    #31674

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog S379A (CDC27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed Serine 379 to Alanine

    Promoter

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

19. GfaABC1D-mGrm3-WPRE

    Plasmid

    #224236

    Purpose

    AAV expression of mGrm3 from GfaABC1D promoter

    Depositor

    Jun Nagai

    Article

    Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

    Insert

    mGrm3 (Grm3 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. GfaABC1D-flag-mAdrb1-WPRE

    Plasmid

    #223671

    Purpose

    AAV expression of flag-mAdrb1 from GfaABC1D promoter

    Depositor

    Jun Nagai

    Article

    Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

    Insert

    mAdrb1 (Adrb1 Mouse)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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