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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,021 results for: D10

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Showing: 981 - 1000 of 2021 results

  1. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112911-plasmid-map-sequence-id-220214

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  4. TFORF0725

    Plasmid
    #143071
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143071-plasmid-map-sequence-id-399051

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  14. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102887
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102887-plasmid-map-sequence-id-262467

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  17. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid
    #189554
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Available Since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189554-plasmid-map-sequence-id-384131

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  18. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid
    #189560
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 220-654
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189560-plasmid-map-sequence-id-384135

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  19. pSCM167-Venus-VF-hADAM10(aa1-459)

    Plasmid
    #189559
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 1-459
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189559-plasmid-map-sequence-id-374396

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Showing: 981 - 1000 of 2021 results