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pAAV-CAG-H2B-GFP (AAV Retrograde)

Viral Prep

#116869-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-H2B-GFP (#116869). In addition to the viral particles, you will also receive purified pAAV-CAG-H2B-GFP plasmid DNA. CAG-driven expression of H2B-GFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Tags

GFP

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only
mNeonGreen-Smad3

Plasmid

#172098

Purpose

Expresses fluorescently tagged human Smad3 in mammalian cells.

Depositor

Lea Goentoro

Article

Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Smad 3 (Smad3 Mouse)

Tags

mNeonGreen

Expression

Mammalian

Mutation

C49S mutation in mCerulean

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-TRAF2

Plasmid

#111202

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP4K2

Plasmid

#23644

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K2 (MAP4K2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-H2B tdTomato (AAV Retrograde)

Viral Prep

#116870-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-H2B tdTomato (#116870). In addition to the viral particles, you will also receive purified pAAV-CAG-H2B tdTomato plasmid DNA. CAG-driven expression of H2B-tdTomato. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Tags

tdTomato

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only
pCR1068

Plasmid

#111092

Purpose

BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Type

Empty backbone

Use

Lentiviral

Mutation

BlpI site disrupted

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-URH49 WT

Plasmid

#110127

Purpose

Expresses Flag-tagged human URH49 (DDX39A)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

April 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTXRE5-Z

Plasmid

#112509

Purpose

Reporter plasmid, responsive to activated Ah receptor/Arnt

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

RW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)

Expression

Yeast

Promoter

5 XREs+ minimal Cytochrome C

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (pGEX2T(TEV))

Plasmid

#80630

Purpose

Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-222

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA C5aR1

Plasmid

#62613

Purpose

Constitutive expression of C5aR1 in mammalian cells

Depositor

Heini Miettinen

Article

Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Insert

C5aR1 (C5AR1 Human)

Expression

Mammalian

Promoter

SRalpha

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223-IPPK

Plasmid

#23415

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IPPK (IPPK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ACVRL1

Plasmid

#23873

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVRL1 (ACVRL1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

GST

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pACBB-eGFP

Plasmid

#32551

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pYLL204

Plasmid

#53455

Purpose

Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Y814F

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-Dicer-delHelicC

Plasmid

#51366

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to HelicC domain deletion

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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