We narrowed to 5,835 results for: pCas

1. pCAGIG-6xHIS.Mm.RPE65-eGFP

   Plasmid

   #41019

   Depositor

   John Nickerson

   Insert

   RPE65 (Rpe65 Mouse)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCap-SG5-Hgy

   Plasmid

   #227580

   Purpose

   Capture plasmid with hygromycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-GFP-CD63

   Plasmid

   #194989

   Purpose

   overexpress CD63

   Depositor

   Angelique Bordey

   Insert

   GFP-CD63
   

   Expression

   Bacterial

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-CBE6c-V106W

   Plasmid

   #215826

   Purpose

   Express CBE6c V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Promoter

   pRSV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_GPHN.FingR-mKate2-IL2RGTC

   Plasmid

   #46297

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-flpE-puro

   Plasmid

   #20733

   Purpose

   Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   FLPe recombinase
   

   Use

   Flp/frt

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAX APP 695

   Plasmid

   #30137

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Expression

   Mammalian

   Mutation

   wild type splice variant 695

   Available Since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mEGFP-CaMKIIa

    Plasmid

    #127389

    Purpose

    Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_hTRPV1-p2A-tdTomato

    Plasmid

    #238923

    Purpose

    Expression of the human TRPV1 channel with tdTomato as a reporter in mammalian cells

    Depositor

    Vsevolod Belousov

    Article

    Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

    Insert

    hTRPV1-p2A-tdTomato
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HA-LMW-FGF2

    Plasmid

    #157663

    Purpose

    Expresses FGF2 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-134

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-EGFP(HiBiT)

    Plasmid

    #162589

    Purpose

    N-terminally HiBiT-tagged EGFP under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    EGFP
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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