We narrowed to 59,942 results for: %s

1. pDEST8_FLAGHA_FUS_HIS

   Plasmid

   #26375

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Tags

   FLAG/HA and His

   Expression

   Insect

   Mutation

   delta 527 (STOP codon)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-N1-Barkor

   Plasmid

   #24402

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Barkor (ATG14 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pCI Flag Smad3 LC EPSM

   Plasmid

   #27038

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 133-425; T179V S204A S208A S213A

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pYES2-gRNA-hyg-MCS

   Plasmid

   #107734

   Purpose

   E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   X-3 site gRNA expression cassettes
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52p

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-RiboJ-LacZa-T7

   Plasmid

   #173224

   Purpose

   pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

   Depositor

   Jim Haseloff

   Article

   Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 9, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PliaG

   Plasmid

   #55174

   Purpose

   Constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   LiaG promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV V5-Tmem214

   Plasmid

   #175153

   Purpose

   Lentiviral expression of V5-tagged mouse Tmem214

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem214 (Tmem214 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI Flag Smad3 NL EPSM

    Plasmid

    #27006

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-226; T179V S204A S208A S213A

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFX-β-tubulin-iRFP

    Plasmid

    #175830

    Purpose

    Express iRFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with microtuble-targeting sequence
    

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-Cas12c2-noncoding

    Plasmid

    #120877

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12c2
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV114

    Plasmid

    #63181

    Purpose

    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1121 pcDNA3 GFP FKHR H215R

    Plasmid

    #9027

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pHAGE2-EF1alpha-HA-TFAP4

    Plasmid

    #110158

    Purpose

    Lentiviral vector for expression of human TFAP4 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTYF-super-mfPV-0.8k-Venus-WPRE

    Plasmid

    #156399

    Purpose

    Expresses Venus under PV gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2 Flag Smad3 S204A

    Plasmid

    #27114

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Mutation

    S204A

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. SNAP-MIDAS

    Plasmid

    #171604

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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