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PX458_NFIL3_2

Plasmid

#86349

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_1

Plasmid

#86348

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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p6993 MSCV-Neo C-HA 18E7

Plasmid

#163311

Purpose

Expresses HPV18 E7

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7

Use

Retroviral

Tags

HA

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 A114Q

Plasmid

#175767

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

A114Q

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF0

Plasmid

#200374

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF1

Plasmid

#200375

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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MTK3_026

Plasmid

#123741

Purpose

Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Flamindo2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HA PRAS40 S183A

Plasmid

#15674

Depositor

John Lawrence

Article

Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

Insert

PRAS40 S183A (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Mutation

S183A

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
p8126 MSCV-P C-FlagHA 16E7 R77S

Plasmid

#163305

Purpose

Expresses HPV16 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV16 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKD282

Plasmid

#125072

Purpose

Expression of SO_4427 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4427 (SO_4427 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVL23 - pVND7

Plasmid

#116019

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pVND7

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
CS2 human OAZ

Plasmid

#14036

Depositor

Joan Massague

Article

Hata et al Cell. 2000 Jan 21. 100(2):229-40.

Insert

OAZ (ZNF423 Human)

Use

Xenopus/mammalian/avian/zebrafish

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
XE7 Xwnt-8

Plasmid

#16869

Depositor

Randall Moon

Article

Christian et al Development. 1991 Apr. 111(4):1045-55.

Insert

Xwnt-8 RNA (wnt8a.L Frog)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

Plasmid

#89071

Purpose

genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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2xFRB-myc

Plasmid

#20227

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only