We narrowed to 13,472 results for: sequence

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pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

Plasmid

#229995

Purpose

PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

Depositor

Christian Mayer

Article

Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

Type

Empty backbone

Use

CRISPR

Tags

NotI site for NEBuilder HiFi DNA Assembly with ss…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA LTB4R

Plasmid

#226810

Purpose

Tet-inducible lentiviral vector for 2xHA LTB4R expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR153

Plasmid

#226808

Purpose

Gateway entry vector for 2xHA GPR153

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-NbON10-NanoLuc

Plasmid

#198691

Purpose

Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, IgK leader sequence, NanoLuc, and twin…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-RhoA*

Plasmid

#198716

Purpose

Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Expression

Mammalian

Mutation

It has mutations that is resistant to the RhoA gR…

Promoter

CAG

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCHC030 (∆CBBp ∆MBH ∆SH)

Plasmid

#226205

Purpose

Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.

Use

Synthetic Biology

Mutation

∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCKC017

Plasmid

#226622

Purpose

pLenti wt hTdT template for libraries 1-2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

synonymous mutation in D2, synonymous mutation in…

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p40651-EFSNS-FMR1_e1ΔCGG-24xms2

Plasmid

#222969

Purpose

MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-ΔCGG 5' UTR and exon1

Expression

Mammalian

Mutation

31 CGG repeats deleted from 5' UTR

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(5p)-WT

Plasmid

#215875

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-WT

Plasmid

#215876

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-WT

Plasmid

#215877

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-WT (MIR589 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(3p)-WT

Plasmid

#215878

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a2(3p)-WT

Plasmid

#215879

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(5p)-I-type

Plasmid

#215882

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-I-type

Plasmid

#215883

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-589(5p)-I-type

Plasmid

#215884

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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