We narrowed to 27,563 results for: STI

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pAf-CRISPR-phoA

Plasmid

#207991

Purpose

CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

phoA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_12xTEAD_luc2

Plasmid

#173004

Purpose

firefly luciferase reporter vector with 12x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

12xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26570

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-beta-actin

Plasmid

#178076

Purpose

Insect cell expression of beta-actin

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

beta-actin (ACTB Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pCD86-mApple

Plasmid

#133859

Purpose

Mammalian expression of CD86 C-terminally tagged with mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 PBD HK-AA (Nigg AH13)

Plasmid

#41161

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD HK-AA (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HA-Arp8

Plasmid

#19796

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

Arp8 (ACTR8 Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMY-MCS-P2A-BlastR-DYK

Plasmid

#163357

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-332

Plasmid

#11625

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino-terminal half of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_BSD_FKBPF36V_EYFP

Plasmid

#163880

Purpose

Acute Depletion of Mau2-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

Plasmid

#101205

Purpose

Bacterial expression plasmid for SpCas9 REC3 domain

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K506–Q712

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K506–Q712

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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