165,839 results

1. TFORF1329

   Plasmid

   #143789

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2A (TFAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-cT3G-cERP2-FOXL2

   Plasmid

   #192914

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2 (FOXL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0144

   Plasmid

   #141536

   Purpose

   Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PHOX2A (PHOX2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-mR2-AAVS1_sgRNA

   Plasmid

   #183890

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-sfGFP

   Plasmid

   #112573

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis, Strep II, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

   Plasmid

   #208377

   Purpose

   Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPapaya-pBAD

    Plasmid

    #54838

    Purpose

    Localization: Protein Expression Vector , Excitation: 530, Emission: 541

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPapaya

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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