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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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EZ-L175

Plasmid

#173025

Purpose

Encodes OptoAMP1 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-YFP-FRB1N

Plasmid

#162431

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB1N

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCx43-7-iRFP670-N1

Plasmid

#197222

Purpose

Expresses the fusion protein of Cx43-7-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-iRFP670

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP720

Plasmid

#197241

Purpose

Expresses the fusion protein of Tubulin-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP720

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHAHA

Plasmid

#12517

Depositor

Tong-Chuan He

Article

Luu et al Cancer Lett. 2005 Nov 8. 229(1):135-48.

Type

Empty backbone

Tags

Double HA tag

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP713-HO1

Plasmid

#197255

Purpose

Expresses the protein of miRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_5a7_pKD46.10

Plasmid

#178937

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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LeGO-hEµMAR-iG2

Plasmid

#52635

Purpose

3rd generation lentiviral vector for enhanced transgene and eGFP-coexpression in primary B cells and B-cell lines

Depositor

Boris Fehse

Article

Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

Insert

hEµMAR-SFFV; IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pH2B-iRFP670-N1

Plasmid

#197227

Purpose

Expresses the fusion protein of H2B-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-iRFP670

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-Actin-C-18

Plasmid

#197221

Purpose

Expresses the fusion protein of miRFP720-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720-Actin-C-18

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-mRhubarb720-N1

Plasmid

#197191

Purpose

Expresses the fusion protein of CytERM-mRhubarb720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-mRhubarb720

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Promoter

Plac

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 specR cassette

Plasmid

#117120

Purpose

Spectinomycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Spectinomycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag7

Plasmid

#175535

Purpose

Expression of HaloTag7 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKM263-ScPPX

Plasmid

#38327

Depositor

Florian Freimoser

Article

Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

Insert

PPX1 (PPX1 Budding Yeast)

Tags

GST, His, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag7

Plasmid

#175531

Purpose

Lyzosomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pXWPrinA-gfp2

Plasmid

#123147

Purpose

pSB1K3 carrying PrinA-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

PrinA-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-H-ttgR

Plasmid

#62565

Purpose

Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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