We narrowed to 13,693 results for: crispr cas9

1. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97311

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-AlbEx14-mNG

   Plasmid

   #201780

   Purpose

   knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

   Depositor

   Hajime Hirase

   Article

   Vittani et al Fluorescence Imaging of the Brain. Neuro

   Inserts

   Alb gRNA (Alb Mouse)
   Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97309

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97310

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307_CR-PDHA2S293A

   Plasmid

   #115204

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307_CR-PDHA2

   Plasmid

   #115199

   Purpose

   Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307_CR-PDHA2S291A

   Plasmid

   #115203

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMYT095

   Plasmid

   #198866

   Purpose

   CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

   Depositor

   Tom Ellis

   Article

   Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRVV651

    Plasmid

    #87631

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMYT096

    Plasmid

    #198867

    Purpose

    Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

    Depositor

    Tom Ellis

    Article

    Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVV647

    Plasmid

    #87630

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_835

    Plasmid

    #245327

    Purpose

    Cas9 CRISPRko positive control guide; targets CD59

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD59 (CD59 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRVV653

    Plasmid

    #89474

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRVV598

    Plasmid

    #87629

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGM51323

    Plasmid

    #153224

    Purpose

    Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
    

    Use

    Synthetic Biology

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Expression

    Bacterial

    Promoter

    lacIq-Ptrc

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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