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pENTR L1-13XLexAop2-R5

Plasmid

#41433

Depositor

Steve Stowers

Insert

13XLexAop2

Expression

Bacterial

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGPDluxAB(Ura)

Plasmid

#1119

Depositor

Susan Lindquist

Article

Singer et al Mol Cell 1998 Apr;1(5):639-48.

Insert

bacterial luciferase

Expression

Yeast

Mutation

n/a

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pTE-mcs

Plasmid

#20320

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

multiple cloning site

Expression

Bacterial

Available Since

March 4, 2009

Availability

Academic Institutions and Nonprofits only

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pCap-101-Hgy

Plasmid

#227508

Purpose

Capture plasmid with hygromycin resistance and pIJ101 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSB223

Plasmid

#164127

Purpose

New measurement standard

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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slc1a3b:mCD8mCherry

Plasmid

#170207

Purpose

Drives mCD8mCherry expression with the slc1a3b promoter in astrocytes

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Promoter

slc1a3b

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCB301TOR

Plasmid

#164798

Purpose

Oomycete expression vector using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

NPTII

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE335

Plasmid

#153244

Purpose

M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE337

Plasmid

#153246

Purpose

M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:EGFP-Mmu.Rpl10a)

Plasmid

#128840

Purpose

RNA isolation from machrophages via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-Sth1Cas9

Plasmid

#129552

Purpose

Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

Sth1Cas9

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Expression

Bacterial

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTK317

Plasmid

#59571

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQ2aB

Plasmid

#124887

Purpose

Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP

Depositor

Nevil Singh

Type

Empty backbone

Use

Retroviral

Tags

Blue Flourescent Protein, Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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OeNL_pRSETb

Plasmid

#89529

Purpose

Orange color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pK

Plasmid

#64188

Purpose

harbours marker gene LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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