We narrowed to 16,367 results for: nans

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pAAV-nEF-Con/Fon/Von eYFP

Plasmid

#137164

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV Retrograde and AAV8

Insert

3x-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NPHS1-COMP5AP-AviTag-9xHis

Plasmid

#157587

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NPHS1 (NPHS1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-EWSR1-repair-tempate

Plasmid

#237682

Purpose

For knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWSR1 (EWSR1 Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX301-ARF6-TagRFP

Plasmid

#162029

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGIRR-COMP5AP-AviTag-9xHis

Plasmid

#157058

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGIRR (SIGIRR Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 KSR2

Plasmid

#25968

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
JAM2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176966

Purpose

Mammalian expression vector encoding JAM2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1586

Plasmid

#121057

Purpose

mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mKate2-T^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized mKate2, and au…

Expression

Worm

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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WT USP7 TRAF

Plasmid

#227226

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 62-205

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-Luc

Plasmid

#122034

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

Luciferase

Use

AAV

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis

Plasmid

#157562

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO1 (ROBO1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOD2048-csnDEG

Plasmid

#89368

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Posm-6

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only
pPC1000

Plasmid

#174829

Purpose

Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Inserts

CRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

EF1a and mouse U6

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Article

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD1B-COMP5AP-AviTag-9xHis

Plasmid

#157404

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD1B (CD1B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis

Plasmid

#157239

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DS1 (KIR2DS1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK-hACE2

Plasmid

#161612

Purpose

Expression of human ACE2 in mammalian cells

Depositor

Brad Rosenberg

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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