222,626 results

1. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLZts

   Plasmid

   #128799

   Purpose

   Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

   Depositor

   Timothy Barnett

   Article

   Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

   Type

   Empty backbone

   Use

   Bacterial mutagenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PEmax-mRNA

   Plasmid

   #204472

   Purpose

   plasmid for PEmax mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

   Insert

   Prime editor
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-VSVP

   Plasmid

   #64088

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV phosphoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-VSVP

   Plasmid

   #64088

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV phosphoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Str-Ii_neomycin

   Plasmid

   #65312

   Purpose

   Cytoplasmic ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Streptavidin in its cytoplasmid part

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Str-Ii_neomycin

   Plasmid

   #65312

   Purpose

   Cytoplasmic ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Streptavidin in its cytoplasmid part

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabe-puro largeTcDNA

   Plasmid

   #14088

   Depositor

   William Hahn

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   Large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge