167,948 results

1. pSEM238

   Plasmid

   #161515

   Purpose

   Neuronal expression of histamine-sensitive chloride channel for negative selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

   Insert

   pEXP[Psnt-1 | histamine | rpl-3 UTR]
   

   Expression

   Worm

   Promoter

   Psnt-1

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti.CAG.H2B-Dendra2.W

   Plasmid

   #51005

   Purpose

   Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

   Depositor

   Rusty Lansford

   Insert

   H2B-Dendra2
   

   Use

   Lentiviral

   Tags

   histone2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

   Plasmid

   #183410

   Purpose

   piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   KRAB-dCas9-ecDHFR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ-Myc-GATA4

   Plasmid

   #232647

   Purpose

   transient expresses GATA4 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. hACE2

   Plasmid

   #1786

   Purpose

   Mammalian expression of human ACE2

   Depositor

   Hyeryun Choe

   Article

   Li et al Nature. 2003 Nov 27. 426(6965):450-4.

   Has Service

   Cloning Grade DNA

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. paavCAG-pre-mGRASP-mCerulean

   Plasmid

   #34910

   Purpose

   pre

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Has Service

   AAV5

   Insert

   presynaptic mGRASP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAXEF-Rap1-WT

   Plasmid

   #32697

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Expression

   Mammalian

   Promoter

   EF promoter

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

9. Tol2-elavl3-GCaMP6s

   Plasmid

   #59531

   Purpose

   Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

   Depositor

   Misha Ahrens

   Article

   Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

   Inserts

   elavl3 promoter (elavl3 Zebrafish)
   GCaMP6s
   

   Available Since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CCR5-Tango

    Plasmid

    #66242

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pXL-BacII-CAG-mCherry

    Plasmid

    #170519

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-mCherry
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPdR

    Plasmid

    #85083

    Purpose

    Expresses putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV5)

    Viral Prep

    #202199-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

15. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1-GfaABC1D-Rpl22-HA

    Plasmid

    #111811

    Purpose

    Express Rpl22HA in astrocytes

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Ribosomal protein L22 (Rpl22 Mouse)
    

    Tags

    HA

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-SKII-3XHA-HALO-NAT

    Plasmid

    #188931

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPHK2 gRNA (BRDN0001146242)

    Plasmid

    #77095

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-PDGFRb-IRES2-EGFP

    Plasmid

    #204354

    Purpose

    Expresses wild-type PDGFRb gene.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS13B^Halo

    Plasmid

    #224584

    Purpose

    codon optimized Halo tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^Halo (VPS13B Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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