172,844 results

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pcDNA-LoxP-mKate-LoxP-EGFP

Plasmid

#167940

Purpose

CMV promoter-LoxP-mKate-LoxP-EGFP

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

LoxP-mKate-LoxP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire-PURO

Plasmid

#203654

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-PPARA

Plasmid

#169019

Purpose

Expression of PPARA driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human ppara (PPARA Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-iCre

Plasmid

#122518

Purpose

Cre expression

Depositor

Kimberly Ritola

Insert

iCre

Use

AAV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ heiCas9

Plasmid

#186929

Purpose

For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

Depositor

Joachim Wittbrodt

Article

Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

Insert

myc-flexible linker-oNLS-Cas9-oNLS

Use

CRISPR

Tags

myc-flexible linker-optimizedNLS and optimizedNLS

Expression

Mammalian

Promoter

sp6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPD0054_pIVTRup_mCherry2

Plasmid

#242545

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

mCherry

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

Plasmid

#85428

Purpose

Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-12

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Vps34

Plasmid

#86749

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Vps34 (PIK3C3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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mSirt1-GFP

Plasmid

#221511

Purpose

Fluorescently tagged mouse Sirt1-GFP

Depositor

Alessandro Ori

Insert

Sirt1 (Sirt1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100842

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-ZoAl-ENT-3xF-ORF

Plasmid

#238484

Purpose

For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1103A

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-CMV-GFP

Plasmid

#83257

Purpose

Adenoviral expression of STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Raptor_1 shRNA

Plasmid

#1857

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Raptor (RPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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