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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Article

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-Rab8A

Plasmid

#228880

Purpose

Expression of His-tagged Rab8A in E. coli

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

RAB8A (RAB8A Human)

Tags

His tag

Expression

Bacterial

Mutation

residues 1-6 and 177-207 missing

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DDX54

Plasmid

#97060

Purpose

Expresses FLAG/HA-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only
pBS CMV NA 3C FLAG SA

Plasmid

#234993

Purpose

Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA

Tags

HRV 3C site, FLAG, Strep-Tactin (SA)

Expression

Mammalian

Mutation

In the Streptavidin coding sequence Glu44Val/Ser4…

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pNA 3C myc scfvHA

Plasmid

#234991

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvHA

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Vcp

Plasmid

#123600

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

valosin containing protein (Vcp Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available Since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNAZeo(-) LAMP2C

Plasmid

#89342

Purpose

Expresses human LAMP-2C in mammalian cells

Depositor

Janice Blum

Article

Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

Insert

Lysosomal-associated membrane protein 2C (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Ccnd2PromoterRegion2(0.5kb)

Plasmid

#228059

Purpose

0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Igf2Promoter

Plasmid

#228060

Purpose

0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Igf2 promoter (Igf2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pETM33_CLIP_CAP_Gly

Plasmid

#178470

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLIP_CAP_Gly (CLIP1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMJA285= pSin-TCRab-CMVp-Puro

Plasmid

#116875

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TCR (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of EF1-a promotor

Promoter

CMV Promotor

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1000-1600

Plasmid

#89101

Purpose

mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1000-1600

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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