We narrowed to 13,693 results for: crispr cas9

1. pEF-XMAS-2xStop

   Plasmid

   #164412

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDT-sgRNA-XMAS-2x

   Plasmid

   #164414

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAct:MS2-p65-HSF

   Plasmid

   #78902

   Purpose

   Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2-p65-HSF
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJM149

   Plasmid

   #134168

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC E118A R129A, E. coli codon optimized, CRISPR…

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJM147

   Plasmid

   #134167

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICSL11057

   Plasmid

   #68253

   Purpose

   Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promote:TaU6+sgRNA HvPM19_1
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAWp9-5

   Plasmid

   #73856

   Purpose

   pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
   

   Use

   Lentiviral

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3_3_TOPO_TA-ms2-VP64

   Plasmid

   #79371

   Purpose

   Recruits VP64 to a compatible gRNA for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWp9-4

    Plasmid

    #73855

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1
    

    Use

    Lentiviral

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pICSL11058

    Plasmid

    #68254

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_TaU6 + sgRNA_HvPM19_3
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDM097

    Plasmid

    #216819

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHDR-PMG (pXK-1650)

    Plasmid

    #208858

    Purpose

    HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P2061

    Plasmid

    #186299

    Purpose

    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

    Inserts

    LexA-HBD-B112
    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    2xLexop-minimalCyc1 and PACT1

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMB204: UbC-GFP-Tg_SAP30L-WPRE

    Plasmid

    #168109

    Purpose

    Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    SAP30L (SAP30L T. guttata)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJM143

    Plasmid

    #134166

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEL081

    Plasmid

    #137890

    Purpose

    iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px330-MRPsg1

    Plasmid

    #87189

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 1

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #1 (RMRP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge