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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,213 results for: grna

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Showing: 10161 - 10180 of 16213 results

  8. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  9. sgAAVS1(A)

    Plasmid
    #85570
    Purpose
    Expresses sgRNA targeting human AAVS1 locus
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    adeno-associated virus integration site 1 (AAVS1 Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. pIK208

    Plasmid
    #65630
    Purpose
    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"
    Depositor
    Iskra Katic
    Publication
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    Use
    CRISPR
    Expression
    Worm
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65630-plasmid-map-sequence-id-111541

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  12. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    James Chappell
    Publication
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174369-plasmid-map-sequence-id-345702

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  14. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

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  20. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Publication
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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Showing: 10161 - 10180 of 16213 results