Addgene

We narrowed to 4,370 results for: 683

Showing: 2001 - 2040 of 4370 results

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pYPQ-dpcoCas9-SunTag

Plasmid

#158410

Purpose

CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_CCDC93

Plasmid

#209687

Purpose

Expresses MAC-tagged CCDC93 in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

CCDC93 (CCDC93 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry_EHMT1_full length

Plasmid

#228810

Purpose

Expression of mouse EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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mKO3-TEAD4

Plasmid

#221217

Purpose

RFP fused TEAD4

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

mKO3-TEAD4 (TEAD4 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 hBAX

Plasmid

#129580

Purpose

CRISPR deletion of human BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAX (BAX Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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tdTomato-Golgi-7

Plasmid

#58100

Purpose

Localization: Golgi, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Golgi (B4GALT1 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pBa.Halo-LDLR

Plasmid

#162717

Purpose

Mammalian expression of human LDLR

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

LDLR (LDLR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
EYFP-Golgi-7

Plasmid

#56590

Purpose

Localization: Golgi, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Golgi (B4GALT1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
GAL FLAG103QDProGFPp303

Plasmid

#15587

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-SUV39h1 ts2

Plasmid

#115881

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts1

Plasmid

#115880

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaLinker

Plasmid

#124958

Purpose

Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of linker region between CC domain and P…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

Cell Division Cycle 42 (CDC42 Human)

Use

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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Lyn-mCherry-GAI(1-92)

Plasmid

#37315

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

Lyn and mCherry

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
FRB_LD 0_CS G_tTA-BFP

Plasmid

#58876

Purpose

MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL 103Q+ProGFPp416

Plasmid

#15583

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pClneoMyc mouse LNX2

Plasmid

#37010

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX2 (Lnx2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
mNeonGreen-Sec24D

Plasmid

#226581

Purpose

Encodes Sec24D protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec24D (Sec24 homolog D) (SEC24D Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-NEMO

Plasmid

#187899

Purpose

Mammalian cell expression of Halo-tagged NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

NEMO (Ikbkg Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3_CRKL-Y207F-V5

Plasmid

#196016

Purpose

Expresses CRKL Y207F, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1MycHis/HIP1

Plasmid

#29590

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYFP-Sec24D

Plasmid

#66614

Purpose

YFP fusion of Sec24D

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24D (SEC24D Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUChW Parkin shRNA Mul1 shRNA

Plasmid

#78523

Purpose

Lentiviral vector encoding two shRNAs and mCherry reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-spCas9-N57

Plasmid

#169920

Purpose

Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-N57

Use

CRISPR

Tags

Expression

Mutation

N57 is the PAI domain of SB100X

Promoter

CAG

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mcherry-Myo 9b R1695M

Plasmid

#134958

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
G protein alpha-s-HaloTag

Plasmid

#66993

Purpose

G protein alpha-s internally tagged with HaloTag and EE epitope

Depositor

Catherine Berlot

Insert

G-alpha-s-EE-HaloTag (Gnas Rat)

Use

Tags

HaloTag flanked by SGGGGSGGGGS on each side was i…

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Cdc42(N17)

Plasmid

#11400

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

cell division cycle 42 (CDC42 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

Plasmid

#134863

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS1422

Plasmid

#12548

Depositor

Robert Sobol

Article

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

human Methylpurine DNA glycosylase (MPG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc mouse LNX1

Plasmid

#37009

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX1 (Lnx1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2 mBAX

Plasmid

#129577

Purpose

CRISPR deletion of murine BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAX (Bax Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2001 - 2040 of 4370 results

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