We narrowed to 17,708 results for: URE

1. pLVX puro TRIM21 C54Y - GFP

   Plasmid

   #116942

   Purpose

   Expression of TRIM21 C54Y -GFP fusion

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   TRIM21 C54Y-GFP (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C54Y

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-TRIM24-C840W

   Plasmid

   #28136

   Depositor

   Michelle Barton

   Article

   Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   A489V and C840W

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pDEST-FRT-TO-GFP-PALB2 KR

   Plasmid

   #71107

   Purpose

   mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   first 8 Lysine residues mutated to Arginine (K14-…

   Promoter

   CMV/TO

   Available Since

   Dec. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Promoter

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCTCON2-GFP-TAG

   Plasmid

   #158131

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Introduced a stop codon in place of the tyrosine …

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

   Plasmid

   #83483

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A

   Promoter

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Nxph4-HA

   Plasmid

   #128102

   Purpose

   Express HA tagged Nxph4

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-1-Skp2 WT

    Plasmid

    #81117

    Purpose

    bacterial expression plasmid of Skp2

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1P shABCB7_2

    Plasmid

    #102969

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_2 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P shABCB7_1

    Plasmid

    #102968

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_1 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

    Plasmid

    #91917

    Purpose

    Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LweCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

    Plasmid

    #25458

    Depositor

    Betty Eipper

    Article

    Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

    Insert

    PAM (Pam Rat)
    

    Use

    Tags

    His6 and PAM signal

    Expression

    Mammalian

    Mutation

    Contains aa 498-820 (PALcc= PAL catalytic core) …

    Promoter

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET28b – E. coli RecE residues 564-868

    Plasmid

    #104534

    Purpose

    Expresses E. coli RecE residues 564-868

    Depositor

    Charles Bell

    Article

    Zhang et al Structure. 2009 May 13;17(5):690-702. do

    Insert

    RecE residues 564-866 (C-terminal nuclease domain)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC032 - RcdCas13a from Rhodobacter capsulatus DE442

    Plasmid

    #91922

    Purpose

    Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcdCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

    Plasmid

    #91920

    Purpose

    Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcsCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

    Plasmid

    #91913

    Purpose

    Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    CaCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge