We narrowed to 15,195 results for: Egf
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Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…ExpressionMammalianPromoterTight TRE promoter and U6Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120
Plasmid#123241PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterPGKAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-ABE8e-V106W
Plasmid#193653PurposeExpression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-TadA*8e-V106W, EGFP
ExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, TadA*8e-V106WPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTF-FLAG
Plasmid#217686PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2516Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
Plasmid#196191PurposeTagging human SOX2 gene with P2A-EGFP at the C-terminusDepositorInsertSOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
UseHomology repair donorMutationG2913A mutation to replace the guide cutting site…Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR1
Plasmid#167000PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR3
Plasmid#167002PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR10
Plasmid#167003PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG
Plasmid#217719PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2522Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-a
Plasmid#108538PurposeExpresses human PCDH7 transcript variant a in mammalian cellsDepositorAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2 SH2-SH3
Plasmid#46442DepositorInsertGrb2 (GRB2 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 1-217PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
hV1aR-GFP
Plasmid#67846Purposemammalian expression of human V1a receptor with GFP tagDepositorAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
hV1b-GFP-1
Plasmid#67847Purposemammalian expression of human V1b receptor with GFP tagDepositorAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-A3A-Y130F
Plasmid#193652PurposeExpression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-hA3A-Y130F, EGFP
ExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130FPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorInsertVAMP associated protein A (VAPA Human)
TagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG
Plasmid#217718PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR2 (CELSR2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2362Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only