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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 91,153 results for: mal

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Showing: 10261 - 10280 of 91153 results

  1. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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  2. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  3. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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  7. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202042
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-1xNLS-[Activ]
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202042-plasmid-map-sequence-id-401647

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  8. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202043
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-[Activ]
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202043-plasmid-map-sequence-id-401604

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  9. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

    Plasmid
    #202044
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-VP64
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202044-plasmid-map-sequence-id-401598

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Showing: 10261 - 10280 of 91153 results