We narrowed to 59,942 results for: %s

1. pSLQ1371-sgYAP-10

   Plasmid

   #121424

   Purpose

   sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41a iGluSnFR E25R

   Plasmid

   #119831

   Purpose

   Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25R
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25R

   Promoter

   T7

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a - Ec-ClpX D144L

   Plasmid

   #111514

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Aspartatic acid 144 to Leucine

   Promoter

   T7

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MDH1-PGK-FLAG-TAT-86-D

   Plasmid

   #35976

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "D" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MDH1-PGK-FLAG-TAT-86-C

   Plasmid

   #35982

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "C" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMflCT-o4

   Plasmid

   #101313

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   cat resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMflT-o1

    Plasmid

    #101309

    Purpose

    Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN21

    Plasmid

    #100106

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN61

    Plasmid

    #100122

    Purpose

    expresses putative OCT4 DBD with a T7 tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN35

    Plasmid

    #100120

    Purpose

    expresses a chimeric OCT4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN31

    Plasmid

    #100116

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN32

    Plasmid

    #100117

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR CMVie-Lifeact-mRuby2 L1-R5

    Plasmid

    #84389

    Purpose

    Multisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-Zeo-NKX2-8

    Plasmid

    #75090

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKO-Alpha-Actinin-19

    Plasmid

    #57831

    Depositor

    Michael Davidson

    Article

    Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP eIF2beta C-term (aa 89-331)

    Plasmid

    #45901

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 aa89-331 (Eif2s2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 89-331

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only