We narrowed to 23,671 results for: CRISPR

1. pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236375

   Purpose

   Overexpression of dFnCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   dFnCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236363

   Purpose

   Overexpression of endAsCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   endAsCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   E174R, S542R, K548R

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236362

   Purpose

   Overexpression of HyperdAsCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdAsCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   E174R, D256R, E321R, E371R

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236361

   Purpose

   Overexpression of dAsCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   dAsCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEB32-CrActin-35S

   Plasmid

   #226708

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRGEB32-CrActin-UBI

   Plasmid

   #226709

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-Solo-miRFP670nano3-H2BC11

   Plasmid

   #227331

   Purpose

   Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-H2BC11

   Plasmid

   #227330

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mStayGold-EFS-Puro-PLK4

   Plasmid

   #227311

   Purpose

   Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Solo-mStayGold-PCNT

    Plasmid

    #227285

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-TP53

    Plasmid

    #227318

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TP53 (TP53 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-PITCh-MAP4

    Plasmid

    #227295

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MAP4 (MAP4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-EZR

    Plasmid

    #227293

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of EZR (EZR Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-CEP192

    Plasmid

    #227288

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CEP192 (CEP192 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-PITCh-ARL13B

    Plasmid

    #227276

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of ARL13B (ARL13B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

    Plasmid

    #224788

    Purpose

    SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crCoV20, crCoV21, crCoV24
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p213-SWITCH-OFF

    Plasmid

    #217888

    Purpose

    Retroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p204-U6-Switch-ON

    Plasmid

    #217883

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDB_162

    Plasmid

    #216092

    Purpose

    Cas9 [Sp] CRISPRi targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA436

    Plasmid

    #216035

    Purpose

    Fragmid fragment: (Cas protein) binds MS2 stem loops

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    MS2_v1.1
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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