We narrowed to 13,693 results for: crispr cas9

1. pICSL11062

   Plasmid

   #68256

   Purpose

   Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter_AtU6-26 + sgRNA_BolC.GA4a-2
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

   Plasmid

   #196971

   Purpose

   Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

   Depositor

   Leopold Parts

   Inserts

   Cas9-RT
   rtTA3G-P2A-eGFP
   

   Expression

   Mammalian

   Promoter

   PGK and TREG3G

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-AAVS1-sg

   Plasmid

   #194721

   Purpose

   px458 with guide RNA that target hAAVS1

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   Cas9 (AAVS1 Streptococcus pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP4

   Plasmid

   #166106

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HCP6

   Plasmid

   #166108

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307_CR-PDHA2S291A/S293A

   Plasmid

   #115205

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Synthetic, Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

   Plasmid

   #194017

   Purpose

   Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(CTRL)
   eGFP
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

    Plasmid

    #194015

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.1)
    eGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV and u6

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

    Plasmid

    #194016

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.2)
    eGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    cmb and u6

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid

    #171995

    Purpose

    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT01

    Plasmid

    #223373

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pADH118-17

    Plasmid

    #91727

    Purpose

    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p305N-30

    Plasmid

    #246313

    Purpose

    Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    PtU6.2c3 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    deletion: -T (-29 from TSS) within TATA

    Promoter

    PtU6.2c3 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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