168,037 results

1. pH2B_mCherry_IRES_neo3

   Plasmid

   #21044

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pH2B
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHLSec2-FN-YPet

   Plasmid

   #65421

   Purpose

   Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

   Depositor

   Harold Erickson

   Article

   Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

   Insert

   human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
   

   Tags

   YPet

   Expression

   Mammalian

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABpuro-BluF

   Plasmid

   #46824

   Purpose

   luciferase reporter for BmalI promoter

   Depositor

   Steven Brown

   Article

   Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

   Insert

   Bmal1 (Bmal1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Bmal1

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNL [NlucP/SRE/Hygro] Vector

   Plasmid

   #236810

   Purpose

   Express SRE response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   SRE
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLV-Golgi eGFP

   Plasmid

   #79809

   Purpose

   To express and target GFP into the Golgi apparatus using lentiviruses

   Depositor

   Pantelis Tsoulfas

   Insert

   Golgi eGFP (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   Fusion of the first 62 amino acids of the human b…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-EEA1 wt

   Plasmid

   #42307

   Depositor

   Silvia Corvera

   Article

   Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

   Insert

   EEA1 (EEA1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-FLEX-Arch-GFP (AAV9)

    Viral Prep

    #22222-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Promoter

    CAG

    Tags

    GFP (Cre-dependent)

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only