167,948 results

1. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lifeact-mCherry

   Plasmid

   #193300

   Purpose

   mammalian transient expression - expression of Lifeact-mCherry

   Depositor

   Klaus Hahn

   Article

   Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

   Insert

   Lifeact
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVTRup

   Plasmid

   #101362

   Purpose

   Highly efficient in vitro transcription. Empty backbone.

   Depositor

   Ángel Raya

   Insert

   5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
   

   Use

   For cloning a gene for in vitro transcription pur…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Cys-3HB-SPYcatcher
   

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-YFP-rictor

   Plasmid

   #73387

   Purpose

   Expresses HA and YFP tagged Rictor

   Depositor

   Jie Chen, Taekjip Ha

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYeDP60-hATP13A2-WT

   Plasmid

   #171377

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6.2_mCherry_Grx1_roGFP2

   Plasmid

   #155045

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MGP M155

    Plasmid

    #26527

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Insert

    miR-155 (Mir155 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1-GfaABC1D-lck-jGCaMP8m (AAV9)

    Viral Prep

    #176760-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8m (#176760). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8m plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    GfaABC1D

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

13. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

    Plasmid

    #187100

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-SF-iGluSnFR
    

    Tags

    mCherry-SL2

    Expression

    Worm

    Promoter

    Ttx-3

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJFRC-MUH

    Plasmid

    #26213

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Expression

    Bacterial

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-NLS-actin-R62D

    Plasmid

    #118381

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    NLS and R62D

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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