We narrowed to 21,210 results for: ATO

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BC-A1-003

Plasmid

#78690

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAct:MS2-p65-HSF

Plasmid

#78902

Purpose

Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-p65-HSF

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-CrxshRNA

Plasmid

#73987

Purpose

Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Crx gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARγ

Plasmid

#119915

Purpose

Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARγ

Expression

Mammalian

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ3-Pcsd-LacZ

Plasmid

#191619

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-triple mutation-pa-GCaMP6f

Plasmid

#67570

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

V115H, L64F, T65S

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Tags

EGFP

Expression

Mammalian

Promoter

hsp70

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCFD3-MS2tail-SAM

Plasmid

#78905

Purpose

Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Aug. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N11_trigger

Plasmid

#132733

Purpose

T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N11_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75201

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat

Plasmid

#101338

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-NP1

Plasmid

#65060

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

* see comments

Promoter

EF1alpha

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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BC-A1-005

Plasmid

#78692

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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