We narrowed to 15,943 results for: ckb

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qTAG-N-Blast-mStayGold

Plasmid

#227251

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT034

Plasmid

#225180

Purpose

Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM371 - ce-unc-119 array integration

Plasmid

#182640

Purpose

Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFlpStop-HDR-UAS-2.1-tdTom

Plasmid

#89148

Purpose

FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

5XUAS, 3XP3-dsRed

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWRS_1001

Plasmid

#192205

Purpose

lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

Depositor

William Sellers

Article

Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and RNAi

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Puro

Plasmid

#207728

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ135B2.0

Plasmid

#167159

Purpose

Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TTmcs

Plasmid

#25755

Purpose

Entry vector with TRE-tight promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pFuse-STOP

Plasmid

#217677

Purpose

Empty vector control plasmid containing a stop codon prior to the Fc encoding region

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Type

Empty backbone

Expression

Mammalian

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMV0021 [15ARE23bp-GFP]

Plasmid

#246420

Purpose

AHR GFP reporter plasmid in a lentiviral backbone

Depositor

Marco Jost

Article

Verma et al bioRxiv [Preprint]. 2025 Sep 15:2025.09.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

AHR responsive minimal promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-MCS-Ds

Plasmid

#102416

Purpose

Empty vector with a multiple cloning site flanked by Ds integration elements

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCap-SG5-Hgy

Plasmid

#227580

Purpose

Capture plasmid with hygromycin resistance and pSG5 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSK9065

Plasmid

#84345

Purpose

Tightly controlled expression of mRFP fusion proteins in S. aureus

Depositor

Neville Firth

Article

Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

Type

Empty backbone

Tags

mRFPmars

Expression

Bacterial

Promoter

Pxyl/tet

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Blast

Plasmid

#207714

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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