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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRX-INU-TEX264-FLAG

Plasmid

#128258

Purpose

Expresses TEX264 in mammalian cells

Depositor

Noboru Mizushima

Publication

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Publication

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Publication

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP(C3)-Nup153-N

Plasmid

#64318

Purpose

GFP-tagged mammalian expression of N-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Publication

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-nEF Con/Foff hChR2(H134R)-EYFP

Plasmid

#55647

Purpose

Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

Short Ef1a

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP HSPA6

Plasmid

#19486

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Publication

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available since

June 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1.D2-NP

Plasmid

#134366

Purpose

Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

Depositor

Julien Hanson

Publication

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

D2-NP (DRD2 Human)

Tags

Flag, natural peptide of nanoluciferase, and sign…

Expression

Bacterial and Mammalian

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Publication

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-MyosinVa-Ctail

Plasmid

#110169

Purpose

Dominant negative effect by competing with MyosinVa C-terminal tail interactions

Depositor

Jose Esteban

Publication

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Va (Myo5a Mouse)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

amino acids 1444-1853

Promoter

CMV

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CD8-mCherry-FRB

Plasmid

#100738

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Publication

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

FRB and mCherry

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-ALK6 wt

Plasmid

#80882

Purpose

mammalian expression of ALK6 WT

Depositor

Aristidis Moustakas

Publication

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Publication

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Mouse Alpha2 delta-2 HA pMT2

Plasmid

#58731

Purpose

expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

Depositor

Annette Dolphin

Publication

Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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