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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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MDIS2/MRH1 P1_pECIA14

Plasmid

#114839

Purpose

Prey vector MDIS2/MRH1 P1_pECIA14 should be used with bait vector MDIS2/MRH1 P1_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G18640 (MRH1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

Plasmid

#122055

Purpose

Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

Depositor

Stephen Tapscott

Publication

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MD(ND2bHLH) (Myod1, Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

MyoD chimeric protein substituted with the NeuroD…

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Publication

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Publication

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 kif26b sgRNA1

Plasmid

#102857

Purpose

Expresses sgRNA targeting mouse Kif26b

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Use

Lentiviral

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 kif26b sgRNA2

Plasmid

#102858

Purpose

Expresses sgRNA targeting mouse Kif26b

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Use

Lentiviral

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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DIMT1_pLX307

Plasmid

#98330

Purpose

Lentiviral expression of DIMT1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

DIMT1 (DIMT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ173(JPUB_006777)

Plasmid

#83834

Purpose

Carries NEW operon 10 final

Depositor

Harry Beller

Publication

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Kuste3336
Kuste3339
Kuste3341

Promoter

op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-NKX2-8

Plasmid

#75080

Purpose

retroviral vector holding human NKX2-8 cDNA

Depositor

David Mu

Publication

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse-MycNV419D-IRES-RFP

Plasmid

#73574

Purpose

Mammalian expression of mouse MycN V419D mutant

Depositor

Martine Roussel

Publication

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Mycn (Mycn Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V419D

Promoter

MSCV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-DUS2L-PSKH1

Plasmid

#69472

Purpose

Expresses DUS2L-PSKH1 fusion gene

Depositor

Axel Hillmer

Publication

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

DUS2L-PSKH1 (PSKH1 Human)

Use

Retroviral

Tags

HA TAG

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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