We narrowed to 13,982 results for: crispr grnas

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Promoter

MSCV

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_EIF2AK2

Plasmid

#106108

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting EIF2AK2 (EIF2AK2 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF2

Plasmid

#106102

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF2 (CELF2 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF1

Plasmid

#106101

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF1 (CELF1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCJH009_proD_Cas12c-sgRNA-GFP_CAM

Plasmid

#183070

Purpose

Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

rpr-1 promoter

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pShHELIX_sgRNA_entry (CJT32)

Plasmid

#181782

Purpose

Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rpsL

Plasmid

#89953

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rpsL gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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