We narrowed to 50,156 results for: des.1

1. pGEMHE:mTREK-1(K271Q)

   Plasmid

   #133270

   Purpose

   X. laevis expression vector. It will generate the mouse TREK-1 channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
   

   Use

   Oocyte expression

   Tags

   Expression

   Mutation

   K271Q

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_SORBS1-1/3

   Plasmid

   #91547

   Purpose

   Protein expression and purification of human SH3 domain construct SORBS1-1/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SORBS1-1/3 (SORBS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1371-sgITGB1-1

   Plasmid

   #121533

   Purpose

   sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB1-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ1371-sgITGB4-1

   Plasmid

   #121535

   Purpose

   sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB4-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. STIMATE(1-240)

   Plasmid

   #71280

   Purpose

   Expresses STIMATE or TMEM110 (1-240) in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   STIMATE (1-240) (TMEM110 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pClneoEGFPC RSF-1

   Plasmid

   #66862

   Purpose

   Express GFP-fused C.elegans RSF-1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   RSF-1 (rsf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S18G, S229T, I403T and F563S mutations in RSF-1 c…

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pPK1E-1-gfp

   Plasmid

   #48133

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pClneoMyc' CST-1

   Plasmid

   #66859

   Purpose

   Express Myc-tagged C.elegans CST-1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   CST-1 (cst-1 Nematode)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S48N, H50R, S257P and V340A mutations in CST-1 co…

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pHH0103_SH3KBP1-1/3

    Plasmid

    #91555

    Purpose

    Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3KBP1-1/3 (SH3KBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5' Del 1

    Plasmid

    #16049

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1372 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pPD95.75-cYAP-1

    Plasmid

    #66857

    Purpose

    Generate transgenic C.elegans with cYAP-1 fused to GFP

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    C.elegans YAP-1 (yap-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    C. elegans 5'UTR

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. RhoA gRNA #1

    Plasmid

    #198718

    Purpose

    Cas9-mediated knockout of RhoA in mammalian cells #1

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. RhoB gRNA #1

    Plasmid

    #198721

    Purpose

    Cas9-mediated knockout of RhoB in mammalian cells #1

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoB (Rhob Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. RhoC gRNA #1

    Plasmid

    #198724

    Purpose

    Cas9-mediated knockout of RhoC in mammalian cells #1

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoC (Rhoc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1-Cdc45

    Plasmid

    #208796

    Purpose

    Xenopus laevis FLAG5-His10-TEV-Cdc45

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    Cdc45 (cdc45.L Frog)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. NO- DSD Sensor 1

    Plasmid

    #199785

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    NO- DSD Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPbuCas13b-gRNA-1

    Plasmid

    #184567

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bmpr2 gRNA#1

    Plasmid

    #163388

    Purpose

    Cas9-mediated knockout of Bmpr2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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