We narrowed to 12,674 results for: nsf

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pAAV.E2.ChETA

Plasmid

#220310

Purpose

Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

Depositor

Matt Rowan

Article

Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

Insert

ChETA

Use

AAV

Expression

Mammalian

Promoter

S5E2

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-TurboID

Plasmid

#227178

Purpose

Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector

Depositor

Junmin Peng

Article

Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

Insert

TurboID

Use

AAV

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Promoter

CaMKIIa_short

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mEGFP-CREB (S133A)

Plasmid

#137010

Purpose

AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mammalian

Mutation

Changes Serine 133 into Alanine

Promoter

hSynapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-3xHA-SAPAP3

Plasmid

#190200

Purpose

Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

3xHA-SAPAP3 (Dlgap3 Mouse)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a-ncOGT-TPR

Plasmid

#190822

Purpose

For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

6x His

Expression

Bacterial

Mutation

C467X. Also codon optimized for expression in E.c…

Promoter

T7

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-VTKS6-GFP

Plasmid

#178714

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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hSyn-somAion-citrine

Plasmid

#192570

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

somAion

Use

AAV

Tags

Citrine - soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX(S306D)-2A-mCherry

Plasmid

#118292

Purpose

Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

Doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Serine 306 changed to Aspartic Acid

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX(S306A)-2A-mCherry

Plasmid

#118291

Purpose

Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

Doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

changed Serine 306 to Alanine

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

Plasmid

#154777

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

102TAA 150TAG in GFP reporter, hybrid PylT with …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

Plasmid

#156163

Purpose

Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated THBS1 (amino acid residues 1-954)

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_UCN1.0

Plasmid

#208668

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTMB400_ZFcharm_Prnp_DPM

Plasmid

#220847

Purpose

AAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB399_ZFcharm_Prnp_SPM

Plasmid

#220846

Purpose

AAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB340_ZFcharm_Kv1_Prnp_DPM

Plasmid

#220843

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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