We narrowed to 19,270 results for: tec
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Plasmid#199265PurposeSingle AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 geneDepositorInsertNmeABE8e
UseAAV and CRISPRTagsNLSExpressionMammalianPromoterU1aAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-p53
Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK5 gRNA (BRDN0001145540)
Plasmid#77542Purpose3rd generation lentiviral gRNA plasmid targeting human CDK5DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRKAG1 gRNA (BRDN0001147838)
Plasmid#77745Purpose3rd generation lentiviral gRNA plasmid targeting human PRKAG1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRKAG1 gRNA (BRDN0001149526)
Plasmid#77746Purpose3rd generation lentiviral gRNA plasmid targeting human PRKAG1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK1 gRNA (BRDN0001147433)
Plasmid#77794Purpose3rd generation lentiviral gRNA plasmid targeting human MARK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK1 gRNA (BRDN0001149223)
Plasmid#77796Purpose3rd generation lentiviral gRNA plasmid targeting human MARK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK4 gRNA (BRDN0001147387)
Plasmid#76728Purpose3rd generation lentiviral gRNA plasmid targeting human MARK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK4 gRNA (BRDN0001147537)
Plasmid#76177Purpose3rd generation lentiviral gRNA plasmid targeting human CDK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LmoCas6-p300
Plasmid#126486PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertLmoCas6-p300
UseCRISPRTagsFlag and Flag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-PSDd1.2-GFP
Plasmid#125581PurposeFlp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.DepositorAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero20
Plasmid#187930PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero20DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MLM3747
Plasmid#49965PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a controlDepositorInsert3x Flag Tet1CDmut HB-6 (HBB Human)
UseTALENTags3x FLAGMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFA345D4
Plasmid#49238PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorPromoterEF1aAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)
Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001145716)
Plasmid#76904Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001145899)
Plasmid#76905Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001148292)
Plasmid#76906Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF10
Plasmid#88556PurposeDonor Vector containing ZNF10 transcription factor, part of the Human TFome CollectionDepositorInsertZNF10 (ZNF10 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MLM3743
Plasmid#49962PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a controlDepositorInsert3x Flag Tet1CDmut HB-5 (HBB Human)
UseTALENTags3x FLAGMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only