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pBT195

Plasmid

#127843

Purpose

clone BE4max CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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ptp4a3_L (TAL 3000)

Plasmid

#35992

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting ptp4a3 (ptp4a3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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TAL3131

Plasmid

#41305

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr2e3-Right (nr2e3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
TAL3130

Plasmid

#41304

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr2e3-Left (nr2e3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF72

Plasmid

#13206

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF72

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1::G-CaMP3

Plasmid

#117422

Purpose

For Calcium imaging, expression of G-CaMP3 under the expression of a Pinx-1 promoter (AIB interneurons)

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

G-CaMP3

Expression

Worm

Promoter

inx-1

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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TAL3563

Plasmid

#47282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stxbp1a-right (stxbp1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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Rab7a cds in pGem-T Easy

Plasmid

#80522

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab7 (rab7a Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab7b cds in pGem-T Easy

Plasmid

#80523

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab7b (rab7b Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab11a cds in pGem-T Easy

Plasmid

#80529

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab11a (rab11a Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV CB6 FFLuc

Plasmid

#35655

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1677

Plasmid

#110118

Purpose

Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBT255_(pCA-GFP4m)

Plasmid

#36877

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

GFP

Expression

Mammalian

Mutation

F64L; S65T; V163A; S175G

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-190

Plasmid

#99664

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain ( 125-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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